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CABRI:ICLC |
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Accession_number |
ICLC HTL99019
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Cell_line_name |
380
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Brief_description |
Species: human; Tissue: peripheral blood; Tumor: leukemia, pre-B cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse
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Description |
Species: human male 15 years old; Tissue: peripheral blood; Tumor: leukemia, pre-B cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse
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Depositor |
Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy
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Bibliographic_reference |
Proc Natl Acad Sci USA 1984;81:7166-7170 [PMID: 6334305
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Morphology |
round, small cells, grown in suspension
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Culture_conditions |
continuous culture; RPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 1-2 x 10^6 cells/ml; split 1:2-1:3; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
EBNA negative
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by HLA-DP beta: confirmed as human by PCR
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Karyology |
(8;14) and (14;18) translocations
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Freezing_medium |
90% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Comments |
the cells are very difficult to culture, grow very slowly and should be frozen at not less than 5 x 10^6 cells/ampoule
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